Journal: Cell Reports Medicine

Article Title: Interferon-γ-stimulated antigen-presenting cancer-associated fibroblasts hinder neoadjuvant chemoimmunotherapy efficacy in lung cancer

doi: 10.1016/j.xcrm.2025.102017

Figure Lengend Snippet: Blockade of PD-L2-RGMB interaction to overcome anti-PD1 resistance both in vivo and in PDTF platforms (A) Diagram of interaction between apCAFs and Treg. (B) Expression of RGMB in GEO: GSE253540 datasets (left). (C) Expression of Foxp3 and RGMB in aTreg (activated Tregs) and rTreg (rested Tregs) either with knockout (KO) of FOXP1 or not in the merged GEO: GSE118595 and GEO: GSE121251 datasets. (D) Expression of RGMB among immune cells from TCGA and LUAD datasets. p values are calculated using Wilcoxon test. (E) Expression of RGMB in CD4 + T cells and CD8 + T cells. Data are representative of three independent experiments. p values are determined using unpaired Student’s t test, and error bars show the mean and SEM. (F) Expression of RGMB in FOXP1 + and FOXP1 − Tregs. Data are representative of three independent experiments. p values are determined using unpaired Student’s t test, and error bars show the mean and SEM. (G) Representative ridge plots of RGMB + cells (upper) and quantification (lower). Data are representative of three independent experiments. p values are determined using unpaired Student’s t test, and error bars show the mean and SEM. (H) Re-analyzation results for Foxp1, Il1r1, and RGMB in Treg, Tconv, or CD8 + T cells in the GEO: GSE211062 assay for transposase-accessible chromatin by sequencing (ATAC-seq) dataset. (I) Tumor growth curves: tumor + anti-PD1 (gray), tumor apCAFs + anti-PD1 (orange), tumor cells + apCAFs + anti-PD-L2 + anti-RGMB (blue), and tumor cells + apCAFs + anti-PD-L2 + anti-RGMB (light blue). Individual and mean tumor volume over time. n = 5 mice per group, representative of three independent experiments. (J) The tumor photos of mice in (I). n = 4 mice per group. (K) The tumor weight of mice in (I). n = 4 mice per group. (L) Representative flow cytometric scatterplots of FOXP3 + Treg and FOXP1 + FOXP3 + Treg in mice tumors in (I) (left) and the quantification of them (right). Data are representative of three different experiments. p values are determined using unpaired Student’s t test, and error bars show the mean and SEM. (M and N) Representative flow cytometric scatterplots of GZMB + CD8 + T cells (M) and FOXP1 + FOXP3 + Tregs (N) in PDTF (left) and the quantification of them (right). For (B)–(C), p value is calculated using unpaired Student’s t test. For (I)–(K), data are representative of three independent experiments, p values are determined using unpaired Student’s t test, and error bars show the mean and SEM. For (L)–(N), data are representative of three independent experiments, and p values are determined using unpaired Student’s t test and error bars show the mean and SEM.

Article Snippet: InVivoMAb anti-mouse RGMb , BioXcell , Cat# BE0400; RRID: AB_3665663.

Techniques: In Vivo, Expressing, Knock-Out, Sequencing

Journal: Cell Reports Medicine

Article Title: Interferon-γ-stimulated antigen-presenting cancer-associated fibroblasts hinder neoadjuvant chemoimmunotherapy efficacy in lung cancer

doi: 10.1016/j.xcrm.2025.102017

Figure Lengend Snippet:

Article Snippet: InVivoMAb anti-mouse RGMb , BioXcell , Cat# BE0400; RRID: AB_3665663.

Techniques: Recombinant, Staining, Purification, In Vivo, Control, Protease Inhibitor, Activation Assay, Membrane, Modification, Bicinchoninic Acid Protein Assay, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Cell Isolation, Sequencing, Negative Control, shRNA, Software